کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10143489 | 1646180 | 2018 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of Kruppel homolog 1 (Kr-h1) in methyl farnesoate-mediated vitellogenesis in the swimming crab Portunus trituberculatus
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کلمات کلیدی
GFPKrüppel homolog 1Kr-h1RNA interferingKLFCtBPPortunus trituberculatusdsRNAVitellogenesisVitellogeninSRCbHLH-PAS - BHLH-PASdouble-stranded RNA - RNA دو رشته ایRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدKrüppel-like factor - عامل کریپال مانندMethoprene-tolerant - متاپرن تحملmethyl farnesoate - متیل فارنزواتRace - مسابقهMET - ملاقات کردJuvenile hormone - هورمون جوانیC-terminal binding protein - پروتئین اتصال دهنده انتهایی Cgreen fluorescent protein - پروتئین فلورسنت سبزsteroid receptor coactivator - گیرنده استروئید گیرنده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Similar to the role of juvenile hormone (JH) in insects, methyl farnesoate (MF), the unepoxidized form of JH III, regulates many developmental processes in crustaceans, such as molting and reproduction. We have previously showed that the JH receptor, Methoprene-tolerant (Met), which is also a candidate receptor for MF, might be involved in the MF-mediated vitellogenesis in the swimming crab Portunus trituberculatus. In this study, the role of Kruppel homolog 1 (Kr-h1), a transcription factor downstream Met in JH signaling, was further investigated. The deduced protein of Pt-Kr-h1 contained seven repeats of zinc finger motifs, similar to Kr-h1s from other crustacean species, but differing from the eight zinc finger motifs found in insect Kr-h1s. MF treatment in vitro induced the expression of Pt-Kr-h1 in hepatopancreas but not ovary, which is similar to the MF-responsive pattern of Pt-Met as previously reported. Moreover, the expression of Pt-Kr-h1 decreased significantly after treating with Pt-Met dsRNA, strongly indicating that the Pt-Kr-h1 might be involved in the Met-mediated MF signaling pathway. RNAi of Pt-Met and Pt-Kr-h1 both led to a decrease in vitellogenin (Vg) expression, and the reduction cannot be rescued by adding MF, suggesting the regulation of vitellogenesis by MF may act through Met and Kr-h1. These results would help to enhance the current understanding of the regulatory mechanism of MF signaling, and provide a vital resource for further research into the evolution of hormonal pathways in arthropods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 679, 30 December 2018, Pages 260-265
Journal: Gene - Volume 679, 30 December 2018, Pages 260-265
نویسندگان
Xi Xie, Mingxin Liu, Qinghua Jiang, Hongkun Zheng, Liang Zheng, Dongfa Zhu,