کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10147259 1646468 2018 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enrichment of midsized RNAs with manganese chloride precipitation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Enrichment of midsized RNAs with manganese chloride precipitation
چکیده انگلیسی
Enrichment of specific RNAs is important for RNA analysis. MnCl2 has been used previously to enrich viroid RNA fractions from total RNA from infected plants. We have expanded this method to show that MnCl2 can enrich single-stranded as well as structured RNAs of 450 nt and below from a total RNA preparation. We have applied this method to map the transcription start sites of a PSTVd transcript from total RNA from yeast under conditions where the RNA was previously undetectable.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 560, 1 November 2018, Pages 56-59
نویسندگان
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