کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10235336 | 45029 | 2015 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptomic analysis of Corynebacterium glutamicum in the response to the toxicity of furfural present in lignocellulosic hydrolysates
ترجمه فارسی عنوان
تجزیه و تحلیل ترانسکتکتومی کورینباکتریوم گلوتامیکوم در پاسخ به سمیت فورفورال موجود در هیدرولیز لجن خالص
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Pretreatment of lignocellulosic biomass by acid hydrolysis generates growth inhibitors such as furfural, 5-hydroxymethylfurfural, and acetic acid. Among the inhibitors, furfural strongly inhibits cell growth, our objective was to identify the furfural stimulon of an amino acid producer Corynebacterium glutamicum using transcriptomic analysis. As a result, 182 up-regulated (2-fold) genes and 81 down-regulated (0.5-fold) genes were identified for all furfural stress conditions (6.5Â mM, 13Â mM, and 20Â mM). Based on the functional categories of CoryneRegNet database, the furfural stimulon was related to genetic responses of oxidative stress, SOS responses, and anaerobic respiratory metabolism. To overcome the furfural toxicity, furfural-responsive efflux-like permease encoded by the cg1661 gene was overexpressed, resulting that the optical density at 600Â nm was 1.54-fold enhanced over the control at 12Â h. This study provides alternative strategy of metabolic engineering for biological detoxification in addition to overexpression of NADH-dependent reductases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 50, Issue 3, March 2015, Pages 347-356
Journal: Process Biochemistry - Volume 50, Issue 3, March 2015, Pages 347-356
نویسندگان
Hong-Sil Park, Youngsoon Um, Sang Jun Sim, Sang Yup Lee, Han Min Woo,