کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1066865 948848 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influences of acute ethanol exposure on locomotor activities of zebrafish larvae under different illumination
ترجمه فارسی عنوان
تأثیرات مواجهه حاد با اتانول بر فعالیت حرکتی لارو گورخرماهی تحت نور مختلف
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• 5&7 dpf larvae demonstrated similar locomotor responses to acute ethanol treatment.
• Acute 1% ethanol potentiated dark-induced hyper-locomotor activities of the larvae.
• Acute 2% ethanol increased the locomotor activities of the larvae in the light.
• Acute 2% ethanol diminished the locomotor responses of the larvae to light changes.
• HPLC analysis unveiled involvement of NE, DA, and 5-HT in ethanol intoxication.

Larval zebrafish present unique opportunities to study the behavioral responses of a model organism to environmental challenges during early developmental stages. The purpose of the current study was to investigate the locomotor activities of AB strain zebrafish larvae at 5 and 7 days post-fertilization (dpf) in response to light changes under the influence of ethanol, and to explore potential neurological mechanisms that are involved in ethanol intoxication. AB strain zebrafish larvae at both 5 and 7 dpf were treated with ethanol at 0% (control), 0.1%, 0.25%, 0.5%, 1%, and 2% (v/v%). The locomotor activities of the larvae during alternating light–dark challenges, as well as the locomotor responses immediately following the light transitions, were investigated. The levels of various neurotransmitters were also measured in selected ethanol-treated groups. The larvae at 5 and 7 dpf demonstrated similar patterns of locomotor responses to ethanol treatment. Ethanol treatment at 1% increased the swimming distances of the zebrafish larvae in the dark periods, but had no effect on the swimming distances in the light periods. In contrast, ethanol treatment at 2% increased the swimming distances in the light periods, but did not potentiate the swimming activity in the dark periods, compared to controls. Differences in the levels of neurotransmitters that are involved in norepinephrine, dopamine, and serotonin pathways were also observed in groups with different ethanol treatments. These results indicated the behavioral studies concerning the ethanol effects on locomotor activities of zebrafish larvae could be carried out as early as 5 dpf. The 1% and 2% ethanol-treated zebrafish larvae modeled ethanol effects at different intoxication states, and the differences in neurotransmitter levels suggested the involvement of various neurotransmitter pathways in different ethanol intoxication states.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Alcohol - Volume 49, Issue 7, November 2015, Pages 727–737
نویسندگان
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