کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10899472 | 1084378 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Curcumin inhibits PhIP induced cytotoxicity in breast epithelial cells through multiple molecular targets
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کلمات کلیدی
DCFDAGPx-1RFUBRCA-1DNA adductOTMHprtH2AXNQO-1GSRDEPCHBSSPhIPHCANrf2CATPARP-12′,7′-dichlorodihydrofluorescein diacetate - 2 '، 7'-dichlorodihydrofluorescein diacetateDMSO - DMSOROS - ROSDNA damage - آسیبDNAHeterocyclic amines - آمین های heterocyclicdiethylpyrocarbonate - دیاتیلپیر کربناتDimethyl sulfoxide - دیمتیل سولفواکسیدnuclear factor (erythroid-derived 2)-like 2 - فاکتور هسته ای (erythroid-derived 2) -like 2FoxO - فاکسوPhytochemicals - فیتوکمیکال هاOlive tail moment - لحظه دم زیتونcyclin-dependent kinase inhibitor 2A - مهارکننده 2A کیناز وابسته به سیکلینhypoxanthine phosphoribosyl transferase - هیپوکسانتین فسفریبوسیل ترانسفرازRelative Fluorescence Units - واحد فلورسانس نسبیforkhead box protein - پروتئین جعبه جعبهCancer prevention - پیشگیری از سرطانCatalase - کاتالازglutathione reductase - گلوتاتیون ردوکتازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), found in cooked meat, is a known food carcinogen that causes several types of cancer, including breast cancer, as PhIP metabolites produce DNA adduct and DNA strand breaks. Curcumin, obtained from the rhizome of Curcuma longa, has potent anticancer activity. To date, no study has examined the interaction of PhIP with curcumin in breast epithelial cells. The present study demonstrates the mechanisms by which curcumin inhibits PhIP-induced cytotoxicity in normal breast epithelial cells (MCF-10A). Curcumin significantly inhibited PhIP-induced DNA adduct formation and DNA double stand breaks with a concomitant decrease in reactive oxygen species (ROS) production. The expression of Nrf2, FOXO targets; DNA repair genes BRCA-1, H2AFX and PARP-1; and tumor suppressor P16 was studied to evaluate the influence on these core signaling pathways. PhIP induced the expression of various antioxidant and DNA repair genes. However, co-treatment with curcumin inhibited this expression. PhIP suppressed the expression of the tumor suppressor P16 gene, whereas curcumin co-treatment increased its expression. Caspase-3 and -9 were slightly suppressed by curcumin with a consequent inhibition of cell death. These results suggest that curcumin appears to be an effective anti-PhIP food additive likely acting through multiple molecular targets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 365, Issue 1, 28 August 2015, Pages 122-131
Journal: Cancer Letters - Volume 365, Issue 1, 28 August 2015, Pages 122-131
نویسندگان
Ashok Jain, Abhilash Samykutty, Carissa Jackson, Darren Browning, Wendy B. Bollag, Muthusamy Thangaraju, Satoru Takahashi, Shree Ram Singh,