کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11003681 1461590 2019 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
[INVITED] Optical imaging and localization of prospective scattering labels smaller than a single protein
ترجمه فارسی عنوان
[دعوت شده] تصویربرداری نوری و محلی سازی برچسب های پراکندگی احتمالی کوچکتر از پروتئین تک
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی برق و الکترونیک
چکیده انگلیسی
Labeling of biological matter is a key technique enabling visualization and real-time tracking of dynamic processes beyond the diffraction limit. While the precision of fluorescence-based localization microscopy is limited by signal saturation, a new generation of small scattering labels (such as gold nanoparticles) have brought the localization precision below the size of a single protein. However, the size of scattering labels is also the most obvious handicap, as they are usually significantly larger than the resulting localization precision. We address this inconsistency and demonstrate detection and precise localization of gold nanoparticles of only 2 nm in diameter using interferometric detection of scattering (iSCAT). This size is considerably smaller than the size of a small protein and in combination with the possibility of tracking the nanoparticle position, we foresee a new concept of detailed studies of biomolecular systems in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Optics & Laser Technology - Volume 109, January 2019, Pages 323-327
نویسندگان
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