کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11005743 1494806 2018 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of vitamin B12 in equine urine by liquid chromatography - inductively coupled plasma - mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of vitamin B12 in equine urine by liquid chromatography - inductively coupled plasma - mass spectrometry
چکیده انگلیسی
Regulating authorities in the racing industry have restricted the administration of potentially performance enhancing cobalt salts to horses. There are severe penalties for trainers presenting horses with elevated urine cobalt concentrations, and compliance is ensured via analysis of total urinary cobalt at thresholds of 100 μg/L. When cobalt is present as part of the cobalamin molecule it is not considered performance enhancing. This paper demonstrates that a horse can excrete a significant proportion of a commercially available vitamin B12 injection in urine without metabolic modification. A liquid chromatography - inductively coupled plasma - mass spectrometry (LC-ICP-MS) method is presented for urinary cobalt speciation. Given the serious nature surrounding performance enhancing drug offences, we conclude that presumptive positives identified by urine total cobalt measurements require further analysis to differentiate inorganic cobalt from vitamin B12.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Trace Elements in Medicine and Biology - Volume 50, December 2018, Pages 634-639
نویسندگان
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