کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11024824 | 1701067 | 2019 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A procedure for indirect shoot organogenesis of Polianthes tuberosa L. and analysis of genetic stability using ISSR markers in regenerated plants
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کلمات کلیدی
thidiazuronTuberoseIndirect organogenesis2iPPGRsTDZIBAISSRBAPNaAIAA2,4,5-T - 2،4،5-T6-Benzylaminopurine - 6-بنزیلامینوپورینMurashige and Skoog - Murashige و Skoog2,4,5-trichlorophenoxyacetic acid - اسید 2،4،5-ترایکلروپنوکسی استاتیکindole-3-butyric acid - اسید Indole-3-Butyricindole-3-acetic acid - اسید Indole-3-استیکPlant growth regulators - تنظیم کننده های رشد گیاهیinter simple sequence repeats - توالی بین توالی تکرار می شودnaphthalene acetic acid - نفتالین استیک اسیدProline - پرولینTissue culture - کشت بافت
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Efficient in vitro regeneration system was well-established in Polianthes tuberosa L., an attractive ornamental and aromatic plant of horticultural importance. Various explants (Crown explants, leaves, petals, flowering stems and bulb scales) and different plant growth regulators (PGRs) were evaluated for both callus induction and shoot regeneration. Results showed that the maximum callus formation was obtained in crown explants using Murashige and Skoog (MS) medium supplemented by 1.95 μM 2,4,5-Trichlorophenoxyacetic acid (2,4,5-T). The highest rate of shoot regeneration frequency was obtained on MS medium enriched with 2.26 μM thidiazuron (TDZ) + 250â¯mgâ¯lâ1 proline from crown callus. The regenerated shoots were elongated on MS medium supplemented with 2.22 μM 6-benzylaminopurine (BAP). All shoots were successfully rooted in MS medium containing 9.89 μM indole-3-butyric acid (IBA) within a 15-day under a 16/8â¯h photoperiod. Plantlets were successfully hardened and adapted in the greenhouse with a survival rate of 100% and demonstrated normal growth. Flow cytometry and Inter Simple Sequence Repeats (ISSR) markers analysis indicated that there was no variation between mother and regenerated plants. The established regeneration protocol would be very useful for genetic engineering of P. tuberosa L. leading to the production of new flower colour and shape, pest and disease resistance and fragrance modification.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 244, 26 January 2019, Pages 315-321
Journal: Scientia Horticulturae - Volume 244, 26 January 2019, Pages 315-321
نویسندگان
Ayoub Molaahmad Nalousi, Abdollah Hatamzadeh, Pejman Azadi, Motahareh Mohsenpour, Habibollah Samizadeh Lahiji,