کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11025839 1666508 2018 42 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Systemic expression of galectin genes in periparturient goats
ترجمه فارسی عنوان
بیان سیستمیک ژنهای گالکتین در بز های پرپراطوری
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی
The goat industry is one of the major sources of food and protein consumed worldwide. The growth of this industry is faced by challenges such as infection and pathogens. Parturition is a very dynamic period. The coincidental occurrence of periparturient immune suppression and a rise in the shedding of parasite eggs pose a challenge for animal health and production. Galectins constitute an evolutionarily conserved family of β-galactoside-binding proteins. They regulate innate and adaptive immunity and homeostasis. Expression of Galectins may regulate periparturient immune suppression. Galectin gene expression was studied in goat blood during the periparturient period. Body weight, body condition and FAMACHA scores, and fecal and blood samples were collected from Five BoerXSpanish goats at 14 days and 7 days after parturition. Fecal samples were used to assess parasite load. Total RNA was isolated from blood using Trizol and converted to cDNA for real-time PCR using specific primers for goat LGALs-1, -2, -3, -4, -7, -8, -9, -11, -12, -14, -15, -16, and ligand Gal3bp, T-cell immunoglobulin domain, and mucin domain 3(TIM-3). Beta-actin and GAPDH housekeeping genes were used as internal controls. Fold changes in transcript abundance were compared to non-pregnant goats and calculated using the Livak method. Secretion of GALS-1, -3 and -9 in plasma was detected using ELISA. Data were analyzed using SAS 9.4 and Pearson correlations (p<0.05). Galectins were expressed and correlated to changes in leukocytes and fecal egg counts. Secreted GALS-1 decreased and GALS-3 and -9 increased (p<0.05) postpartum. Differential expression of Gal may have functional implications in animal health and homeostasis and needs further study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Small Ruminant Research - Volume 168, November 2018, Pages 60-68
نویسندگان
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