کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11263548 | 1666508 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of season on intrafollicular fatty acid concentrations and embryo production after in vitro fertilization and parthenogenic activation of prepubertal goat oocytes
ترجمه فارسی عنوان
تاثیر فصل بر غلظت اسید چرب داخل فولیکولیک و تولید جنین پس از لقاح داخل وریدی و فعالیت پتاژنوژنیک تخمک های بز بره گوسفند
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
In this study we have assessed the concentration of 13 fatty acids (FAs) in follicular fluid (FF) and tested the blastocyst development of prepubertal goat oocytes after in vitro fertilization (IVF) and parthenogenic activation (PA) during spring, summer, autumn and winter. Ovaries were collected from a local slaughterhouse and transported to the laboratory during the four seasons. Samples of FF were recovered from follicles and FAs were analyzed by gas chromatography. Oocytes were in vitro matured in TCM-199 with hormones, epidermal growth factor and 10% of fetal bovine serum for 24âh. After maturation, oocytes were inseminated with fresh semen capacitated with heparin or parthenogenically activated with ionomycin and 6-dimetylaminopurine. Zygotes from both groups were cultured in SOF medium for 8 days. The results showed that the polyunsaturated FA (PUFA) concentration of n3 was significantly reduced in autumn compared to winter (46.17 μM and 116.45 μM, respectively) and the n6 to n3 (n6:n3) PUFA ratio was significantly higher in autumn than in winter (11.17 μM vs 4.23 μM, respectively). Blastocyst production was significantly higher (Pâ<â0.05) in winter than autumn after IVF (16.8% and 5.5%, respectively) and showed a statistical tendency (Pâ<â0.1) after PA (22.7% and 11.5%, respectively). In conclusion, in ovaries of prepubertal goats we have observed higher concentrations of n3 PUFAs and lower n6:n3 PUFA ratio in the follicular fluid and oocytes with higher competence to develop up to blastocyst in winter compared to autumn. Moreover, spring, which is a non-breeding season in goat, have not affected in vitro blastocyst production from suckling kid oocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Small Ruminant Research - Volume 168, November 2018, Pages 82-86
Journal: Small Ruminant Research - Volume 168, November 2018, Pages 82-86
نویسندگان
Maria-Gracia Catala, Montserrat Roura, Sandra Soto-Heras, Irene Menéndez, Ignacio Contreras-Solis, Maria-Teresa Paramio, Dolors Izquierdo,