کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11263804 | 1646183 | 2018 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic analysis of lipopolysaccharide activated human monocytes
ترجمه فارسی عنوان
تجزیه و تحلیل پروتئومیک از مونوسیتهای انسانی فعال شده توسط لیپوپ پلی ساکارید
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کلمات کلیدی
LPSDAMPsHMGB1IL-1RAPAMPsSDCV-ATPaseTLRUPLC-MS/MS - UPLC-MS / MSIL-1 receptor antagonist - آنتاگونیست گیرنده IL-1Toll-like receptor - تیالآرtransporter associated with antigen processing - حمل کننده همراه با پردازش آنتی ژنdanger associated molecular patterns - خطرات مولکولی مرتبط با خطرSodium deoxycholate - سدیم دگزیکسولاتendoplasmic reticulum - شبکه آندوپلاسمی TAP - ضربه زدنMass spectrometry - طیف سنجی جرمیlipopolysaccharide - لیپوپلی ساکاریدMonocyte - مونوسیتpathogen associated molecular patterns - پاتوژن الگوی مولکولی مرتبط استProteome - پروتئومHigh mobility group box 1 - کادر تحرک بالا 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
چکیده انگلیسی
Monocytes are key mediators of innate immunity and comprise an important cellular defence against invading pathogens. However, exaggerated or dysregulated monocyte activation can lead to severe immune-mediated pathology such as sepsis or chronic inflammatory diseases. Thus, detailed insight into the molecular mechanisms of monocyte activation is essential to understand monocyte-driven inflammatory pathologies. We therefore investigated the global protein changes in human monocytes during lipopolysaccharide (LPS) activation to mimic bacterial activation. Purified human monocytes were stimulated with LPS for 17âh and analyzed by state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS). The label-free quantitative proteome analysis identified 2746 quantifiable proteins of which 101 had a statistically significantly different abundance between LPS-stimulated cells and unstimulated controls. Additionally, 143 proteins were exclusively identified in either LPS stimulated cells or unstimulated controls. Functional annotation clustering demonstrated that LPS, most significantly, regulates proteasomal- and lysosomal proteins but in opposite directions. Thus, seven proteasome subunits were upregulated by LPS while 11 lysosomal proteins were downregulated. Both systems are critically involved in processing of proteins for antigen-presentation and together with LPS-induced regulation of CD74 and tapasin, our data suggest that LPS can skew monocytic antigen-presentation towards MHC class I rather than MHC class II. In summary, this study provides a sensitive high throughput protein analysis of LPS-induced monocyte activation and identifies several LPS-regulated proteins not previously described in the literature which can be used as a source for future studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 103, November 2018, Pages 257-269
Journal: Molecular Immunology - Volume 103, November 2018, Pages 257-269
نویسندگان
Mads Lausen, Thomas B.G. Poulsen, Gunna Christiansen, Kenneth Kastaniegaard, Allan Stensballe, Svend Birkelund,