کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163571 1490945 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An enzyme-free strategy for ultrasensitive detection of adenosine using a multipurpose aptamer probe and malachite green
ترجمه فارسی عنوان
استراتژی رایگان آنزیمی برای تشخیص حساسیت بالای آدنوزین با استفاده از پروب انتگرام چند منظوره و سبز مالاشی
کلمات کلیدی
پروب انتگرال چند منظوره آدنوزین، سبز مالاشیفت، تشخیص حساسیت، نمونه های ادرار و خون
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A novel multipurpose aptamer probe for adenosine (MAAP) was assembled.
• The use of such a MAAP can detect adenosine at concentration as low as 23 pM.
• The MAAP can also be used for fluorescence or spectrophotometric assay of adenosine.
• The conformational switching mechanism of MAAP by adenosine was verified.

We report on an enzyme-free and label-free strategy for the ultrasensitive determination of adenosine. A novel multipurpose adenosine aptamer (MAAP) is designed, which serves as an effective target recognition probe and a capture probe for malachite green. In the presence of adenosine, the conformation of the MAAP is converted from a hairpin structure to a G-quadruplex. Upon addition of malachite green into this solution, a noticeable enhancement of resonance light scattering was observed. The signal response is directly proportional to the concentration of adenosine ranging from 75 pM to 2.2 nM with a detection limit of 23 pM, which was 100–10,000 folds lower than those obtained by previous reported methods. Moreover, this strategy has been applied successfully for detecting adenosine in human urine and blood samples, further proving its reliability. The mechanism of adenosine inducing MAAP to form a G-quadruplex was demonstrated by a series of control experiments. Such a MAAP probe can also be used to other strategies such as fluorescence or spectrophotometric ones. We suppose that this strategy can be expanded to develop a universal analytical platform for various target molecules in the biomedical field and clinical diagnosis.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 887, 5 August 2015, Pages 179–185
نویسندگان
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