کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1172675 1491340 2016 6 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
A novel procedure for the assessment of the antioxidant capacity of food components
ترجمه فارسی عنوان
یک روش جدید برای ارزیابی ظرفیت آنتی اکسیدانی ترکیبات غذایی
کلمات کلیدی
آنتی اکسیدانی، استرس اکسیداتیو؛ كربناته شدن؛ فلورسانس الکتروفورززروسیکس، گونه های اکسیژن واکنشی؛ DPPH، 2،2-دیفنیل-1-پریکیل هیدرازیل؛ ORAC، ظرفیت جذب رادیکال اکسیژن؛ FBS، سرم جنین گاو؛ H2O2، پراکسید هیدروژن؛ DMSO، dimethy
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Carbonylation, an oxidative modification of the amino group of arginine and lysine residues caused by reactive oxygen species, has emerged as a new type of oxidative damage. Protein carbonylation has been shown to exert adverse effects on various protein functions. Recently, the role of food components in the attenuation of oxidative stress has been the focus of many studies. Most of these studies focused on the chemical properties of food components. However, it is also important to determine their effects on protein functions via post-translational modifications. In this study, we developed a novel procedure for evaluating the antioxidant capacity of food components. Hydrogen peroxide (H2O2)-induced protein carbonylation in HL-60 cells was quantitatively analyzed by using fluorescent dyes (Cy5–hydrazide dye and IC3–OSu dye), followed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and fluorescence determination. Among a panel of food components tested, quinic acid, kaempferol, saponin, squalene, trigonelline, and mangiferin were shown to be capable of suppressing protein carbonylation in HL-60 cells. Our results demonstrated that this fluorescence labeling/SDS–PAGE procedure allows for the detection of oxidative stress-induced protein carbonylation with high sensitivity and quantitative accuracy. This method should be useful for the screening of new antioxidant food components as well as the analysis of their suppression mechanism.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 507, 15 August 2016, Pages 7–12
نویسندگان
, , , , , ,