کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1172939 961636 2016 3 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Housekeeping proteins: How useful are they in skeletal muscle diabetes studies and muscle hypertrophy models?
ترجمه فارسی عنوان
پروتئین های خانه داری: چگونه آنها در مطالعات دیابت عضله اسکلتی و مدل های هیپرتروفی عضله مفید هستند؟
کلمات کلیدی
بتا اکتین؛ آلفا توبولین؛ گاما توبولین؛ آلفا اکتینین؛
β-Actin; α-Tubulin; γ-Tubulin; α-Actinin; Glyceraldehyde-3-phosphate dehydrogenase; Ponceau SPVDF, polyvinylidene difluoride; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; EDL, extensor digitorum longus; CEUA–USP, Institute of Biomedical Sciences at th
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

The use of Western blot analysis is of great importance in research, and the measurement of housekeeping proteins is commonly used for loading controls. However, Ponceau S staining has been shown to be an alternative to analysis of housekeeping protein levels as loading controls in some conditions. In the current study, housekeeping protein levels were measured in skeletal muscle hypertrophy and streptozotocin-induced diabetes experimental models. The following housekeeping proteins were investigated: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-actin, α-tubulin, γ-tubulin, and α-actinin. Evidence is presented that Ponceau S is more reliable than housekeeping protein levels for specific protein quantifications in Western blot analysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 504, 1 July 2016, Pages 38–40
نویسندگان
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