کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1175303 961795 2016 3 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Substrate independent ATPase activity may complicate high throughput screening
ترجمه فارسی عنوان
بسترهای فعالیت ATPase مستقل ممکن است غربالگری توان عملیاتی بالا را پیچیده تر کند
کلمات کلیدی
مالاکیت سبز؛ غربالگری با توان عملیاتی بالا؛
Malachite green; High throughput screening; Substrate-independent ATPase activityCAIR, 4-carboxy-5-aminoimidazole ribonucleotide; HTS, high throughput screening; MGA, malachite green assay; PurC, succinoaminoimidazolecarboxamide ribonucleotide synthethase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Inorganic phosphate release, [Pi], is often measured in an enzymatic reaction in a high throughput setting. Based on the published mechanism, we designed a protocol for our screening for inhibitors of SAICAR synthetase (PurC), and we found a gradual increase in [Pi] in positive control samples over the course of the day. Further investigation indicated that hydrolysis of ATP catalyzed by PurC, rather than substrate-related phosphate release, was responsible for a partial contribution to the signals in the control samples. Thus substrate-independent ATPase activity may complicate high throughput screening.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 510, 1 October 2016, Pages 18–20
نویسندگان
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