کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1200257 1493479 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of amphetamines in hair by integrating sample disruption, clean-up and solid phase derivatization
ترجمه فارسی عنوان
تعیین آمفتامین در مو با یکپارچه سازی اختلال نمونه، پاک سازی و مشتق شدن فاز جامد
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A simplified method for amphetamines in hair by liquid chromatography is proposed.
• Matrix solid phase dispersion (MSPD) is used for sample disruption.
• Solid phase derivatization is carried out on the sorbent used for MSPD.
• The method has been used for the determination of the analytes at low ng/mg levels.

The utility of matrix solid phase dispersion (MSPD) for the direct analysis of amphetamines in hair samples has been evaluated, using liquid chromatography (LC) with fluorescence detection and precolumn derivatization. The proposed approach is based on the employment of MSPD for matrix disruption and clean-up, followed by the derivatization of the analytes onto the dispersant-sample blend. The fluorogenic reagent 9-fluorenylmethyl chloroformate (FMOC) has been used for derivatization. Different conditions for MSPD, analyte purification and solid phase derivatization have been tested, using amphetamine (AMP), methamphetamine (MET), ephedrine (EPE) and 3,4-methylenedioxymethamphetamine (MDMA) as model compounds. The results have been compared with those achieved by using ultrasound-assisted alkaline digestion and by MSPD combined with conventional solution derivatization. On the basis of the results obtained, a methodology is proposed for the analysis of amphetamines in hair which integrates sample disruption, clean-up and derivatization using a C18 phase. Improved sensitivity is achieved with respect to that obtained by the alkaline digestion or by the MSPD followed by solution derivatization methods. The method can be used for the quantification of the tested amphetamines within the 2.0–20.0 ng/mg concentration interval, with limits of detection (LODs) of 0.25–0.75 ng/mg. The methodology is very simple and rapid (the preparation of the sample takes less than 15 min).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1447, 20 May 2016, Pages 47–56
نویسندگان
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