کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1211825 1494027 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous assessment of endogenous thiol compounds by LC–MS/MS
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous assessment of endogenous thiol compounds by LC–MS/MS
چکیده انگلیسی


• We develop a mass spectrometry-based method for simultaneous assessment of thiol compounds (GSH, CysGly, Hcy, Cys, and NAC).
• The approach is sensitive enough to detect thiols contents in vivo.
• The technique tremendously increases detection selectivity.
• Chemical derivatization dramatically increases the stability of thiol compounds.

Biological thiol compounds are very important molecules that participate in various physiological events. Alteration in levels of endogenous thiols has been suggested as a biomarker of early stage of pathological changes. We reported a chemical derivatization- and LC–MS/MS-based approach to simultaneously determine thiol compounds including glutathione (GSH), cysteine (Cys), N-acetyl cysteine (NAC), homocysteine (Hcy), and cysteinylglycine (CysGly) in biological samples. Thiol-containing samples were derivatized with monobromobimane (mBrB) at room temperature, followed by LC–MS/MS analysis. Assessment of the analytes with baseline separation was completed within 10 min, using a gradient elution on a C18 reversed-phase column. Excellent linearity was observed for all analytes over their concentration ranges of 1–400 μM. The lowest limits of detection (S/N = 3) in a range from 0.31 fmol (for NAC) to 4.98 fmol (for CysGly) were achieved. The results indicate that this approach was sensitive, selective, and well suited for high-throughput quantitative determination of the biological thiols.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1029–1030, 1 September 2016, Pages 213–221
نویسندگان
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