کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1218188 1494497 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorometric method based on molecular recognition solid-phase extraction for determination of riboflavin in milk and infant formula
ترجمه فارسی عنوان
روش فلورومتریک بر اساس تشخیص مولکولی در استخراج جامد فاز برای تعیین ریبوفلاوین در شیر و شیر مادر
کلمات کلیدی
استخراج فاز جامد، پلیمر مونوآموخته ریبوفلاوین، شیر، فرمول نوزاد تجزیه و تحلیل بالا، میکرو پلاست، فلوریمتری، ترکیب غذا / تجزیه و تحلیل مواد غذایی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Solid-phase extraction based on molecular recognition of riboflavin.
• Direct determination of riboflavin in the eluate, adapted to microscale analysis.
• Minimum sample treatment (without acidification or protein precipitation).
• High-throughput method, useful for quality control purposes and data compilation.

Riboflavin (vitamin B2) is involved in several biological processes, particularly in energy production, and it is acquired from food ingestion, principally from supplemented food during the first years of life. Therefore, a simple, fast and cost-effective high-throughput method for determination of riboflavin in milk and infant formula is proposed, based on selective extraction using commercially available molecularly imprinted polymers targeted to riboflavin, followed by direct fluorometric determination. Several aspects were studied, namely microplate assay conditions, the composition of eluting solution and the stability of riboflavin in the eluate. Hence, elution using 1% (v/v) acetic acid in methanol or in acetonitrile is recommended, followed by immediate analysis or solvent evaporation, with reconstitution and analysis within 24 h. The proposed method provided a LOD of 0.03 mg L−1, with working range for undiluted samples between 0.125 and 2 mg L−1, and sample throughput of 24 h−1. It was successfully applied to certified reference material NIST-1846 and also to commercial milk and infant formula samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Composition and Analysis - Volume 45, February 2016, Pages 141–146
نویسندگان
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