کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1219541 1494543 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Flaxseed secoisolariciresinol diglucoside and enterolactone down-regulated epigenetic modification associated gene expression in murine adipocytes
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Flaxseed secoisolariciresinol diglucoside and enterolactone down-regulated epigenetic modification associated gene expression in murine adipocytes
چکیده انگلیسی


• The effects of flaxseed lignan on epigenetic modification associated gene expression were determined.
• Reactive oxygen species up-regulate epigenetic modification associated gene expression in murine adipocytes.
• Flaxseed lignan down-regulates epigenetic modification associated gene expression in murine adipocytes.

This study was conducted to determine flaxseed secoisolariciresinol diglucoside (SDG) and its metabolite (enterolactone, EL) effects on DNA methyltransferase (DNMT), histone deacetylase (HDAC), and methyl-CpG-binding domain protein (MBD) expression in reactive oxygen species treated murine adipocytes. SDG or EL treated adipocytes (1 × 107) were treated with hydroxyl or superoxide radical, and then total RNA was extracted and purified using an RNeasy Mini kit. DNMTs, HDACs, and MBD2 expression by real-time quantitative PCR was measured. The fold change in expression of the DNMTs, HDACs, and MBD2 was calculated using 2-ΔΔCT method. The results showed that flaxseed SDG and enterolactone downregulate DNMTs, HDACs, and MBD2 expression. How DNMTs, HDACs, and MBD2 expression was down-regulated by flaxseed SDG and enterolactone is unknown and needs further study.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 23, May 2016, Pages 523–531
نویسندگان
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