کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1219910 1494549 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sulforaphane protects human umbilical vein cells against lipotoxicity by stimulating autophagy via an AMPK-mediated pathway
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sulforaphane protects human umbilical vein cells against lipotoxicity by stimulating autophagy via an AMPK-mediated pathway
چکیده انگلیسی


• Sulforaphane protects human umbilical vein cells against lipotoxicity.
• Sulforaphane alleviates lipotoxicity via stimulating autophagy.
• Sulforaphane stimulates autophagy via an AMPK-mediated pathway.
• NRF2 is not involved in the protective role of sulforaphane.

The protective role of sulforaphane (SFN) against saturated fatty acid (SFA)-induced lipotoxicity in human umbilical vein endothelium cell was determined. SFN protected endothelium cells against SFA-induced cell death. This protective activity was Nrf2-independent because silencing Nrf2 did not block the protective action of SFN. Subsequent studies revealed that SFN-induced autophagy contributed to the protective role. Incubation of the cells with autophagy inhibitors significantly blocked the protection by SFN. In addition, SFN significantly enhanced the formation of LC3-II and increased autophagic flux. Mechanistic study revealed that SFN significantly stimulated the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and reduced the phosphorylation of the mammalian target of rapamycin (mTOR). Moreover, knocking-down AMPK significantly blocked SFN-induced autophagy and inhibited its protective role against lipotoxicity. This finding provides novel data demonstrating that SFN protects endothelial cells from SFA-induced lipotoxicity through activating autophagy via an AMPK/mTOR involved pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 15, May 2015, Pages 23–34
نویسندگان
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