کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1220206 1494611 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HPLC method development for the online-coupling of chromatographic Perilla frutescens extract separation with xanthine oxidase enzymatic assay
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
HPLC method development for the online-coupling of chromatographic Perilla frutescens extract separation with xanthine oxidase enzymatic assay
چکیده انگلیسی


• Continuous flow assay to monitor enzymatic regulation.
• Active compounds in Perilla frutescens.
• HPLC separation (and resulting logD distribution) in combination with enzyme assay.
• Mass spectrometric detection.
• Strategy to identify unknown compounds by physico-chemical parameter and literature search.

Enzyme-regulatory effects of compounds contained in complex mixtures can be unveiled by coupling a continuous-flow enzyme assay to a chromatographic separation. A temperature-elevated separation was developed and the performance was tested using Perilla frutescens plant extracts of various polarity (water, methanol, ethanol/water). Owning to the need of maintaining sufficient enzymatic activity, only low organic solvent concentrations can be added to the mobile phase. Hence, to broaden the spectrum of eluting compounds, two different organic solvents and various contents were tested. The chromatographic performance and elution was further improved by the application of a moderate temperature gradient to the column. By taking the effect of eluent composition as well as calculated logD values and molecular structure of known extract compounds into account, unknown features were tentatively assigned. The method used allowed the successful observation of an enzymatic inhibition caused by P. frutescens extract.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 124, 30 May 2016, Pages 347–357
نویسندگان
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