In vitro and in vivo evidence for a satiating effect of fish protein hydrolysate obtained from blue whiting (Micromesistius poutassou) muscle

To find appetite suppressive molecules derived from fish protein hydrolysates, both in vitro and in vivo experiments were performed in order to demonstrate that hydrolysates produced from blue whiting muscle (BWMH) possess satiating properties. Here we demonstrated for the first time that a protein hydrolysate obtained from marine source was able to enhance cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) secretion in STC-1 cell line. To demonstrate that these in vitro activities also exist in vivo, we investigated the effect of BWMH preload administration in rats and its repercussion on food intake and metabolic plasma marker levels. Results showed that BWMH reduced the short term food intake which was correlated to an increase in the CCK and GLP-1 plasma levels. Moreover it was demonstrated that the chronic administration of BWMH led to a decrease in the body weight gain.

► Blue whiting muscle hydrolysate (BWMH) possesses satiating properties.
► BWMH was able to enhance CCK and GLP-1 secretion in STC-1 cell line.
► BWMH reduced the short term food intake in rats.
► This reduction was correlated to an increase in the CCK and GLP-1 plasmatic levels.