کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1220568 1494615 2016 7 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Simultaneous enantioseparation and purity determination of chiral switches of amlodipine and atenolol by liquid chromatography
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موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous enantioseparation and purity determination of chiral switches of amlodipine and atenolol by liquid chromatography
چکیده انگلیسی


• First report describing the simultaneous determination of amlodipine and atenolol enantiomers by direct chiral HPLC.
• Systematic assessment of effects of individual components of mobile phase and their interaction on enantioseparation was evaluated using Box–Behnken design.
• Robustness was tested using Plackett–Burman design of experiments and Robust domains were plotted using contour profiling.
• Applicability of method was tested by analyzing three different commercially available enantiopure formulations.
• Obtained results demonstrated the suitability of proposed method in routine enantiopurity control of chiral switches of amlodipine and atenolol.

A novel, selective and robust enantiospecific HPLC method was developed for simultaneous determination of amlodipine and atenolol enantiomers. Box–Behnken design was employed to identify the effect of factors (% ethanol, % diethylamine and flow rate) and their interactions on enantioresolution and analysis time. Chromatography was performed using mobile phase comprising acetonitrile, ethanol and DEA (92:8:0.2% v/v/v) delivered at a flow rate of 1.2 mL min−1 on a Lux Cellulose-4 column. The enantiomers were monitored at a wavelength of 240 nm and separation was achieved within 8 min. The method was validated in terms of specificity, linearity, accuracy, precision, limit of detection and quantification. The method was found to be linear (R2 ≥ 0.991), accurate (99.8–101.4%) and precise (%RSD ≤ 3%). Additionally, fractional factorial design was used to evaluate the robustness of the method and non-significant intervals for mixture related factors were established using contour profiling. Furthermore, the pertinence of this validated method was established by analyzing three different commercially available formulations. The obtained results confirmed that the proposed method can be extended for routine enantiopurity assay of amlodipine and atenolol in pharmaceutical formulations.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 120, 20 February 2016, Pages 221–227
نویسندگان
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