کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1221667 1494639 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of assay type and sample matrix on detected cytokine concentrations in human blood serum and nasal lavage fluid
ترجمه فارسی عنوان
اثر نوع آزمون و ماتریس نمونه بر روی غلظت های سیتوکین شناسایی شده در سرم خون انسان و مایع لارو بینی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• IL-6 and IL-8 concentrations analyzed with multi- and singleplex platforms were compared.
• Correlation of nine cytokines in nasal lavage fluid and serum was analyzed.
• Results from single and multiplex assays were concordant but not equal.
• Cytokine concentrations in serum do not correspond with levels in nasal lavage fluid.

Cytokine concentrations in biological fluids are widely used markers for activation of immunological processes. Confirming the reproducibility of measurements is important, especially in longitudinal or multicenter studies where time between analyses or different analyzing laboratories increases the intra-assay variability. In this study, the reproducibility of the cytokine analysis conducted with different assay platforms was studied by comparing the results of two cytokines [interleukin (IL)-6 in serum and nasal lavage fluid (NAL) and IL-8 in NAL] analyzed with Meso Scale Discovery (MSD) ultra-sensitive single and multiplex assay kits (n = 76). In addition, the difference in cytokine levels between two biological sample matrices was studied by comparing the results of altogether 9 cytokines [IL-6, IL-2, IL-8, IL12p70, IL-1β, granulocyte–macrophage colony-stimulating factor (GM-CSF), interferon (IFN)γ, IL-10 and tumor necrosis factor (TNF)α] measured from serum and NAL of the same study subjects (n = 460).The results show that the cytokine concentrations analyzed with single and multiplex assays are concordant but not equal. Comparison of the different matrices revealed that cytokine concentrations in serum do not correspond with concentrations detected in nasal lavage fluid.It can be concluded that comparability of the results from single and multiplex analysis of cytokines is high, but the concentrations should not be compared directly with each other. The differences between concentrations analyzed from serum and nasal lavage fluid indicate that the levels are specific for each matrix and represent distinct immunological conditions.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 96, 5 August 2014, Pages 151–155
نویسندگان
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