کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1222413 967863 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determination of efavirenz, rifampicin and its metabolite desacetyl rifampicin levels in human plasma
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous determination of efavirenz, rifampicin and its metabolite desacetyl rifampicin levels in human plasma
چکیده انگلیسی

A simple and rapid isocratic, high performance liquid chromatography (HPLC) assay employing solid phase extraction (SPE) for the simultaneous determination of the anti HIV drug, efavirenz, the anti-tuberculosis drug, rifampicin and the desacetyl metabolite of rifampicin in plasma from HIV/tuberculosis infected patients has been developed. Using a Zorbax SB-Phenyl reverse-phase analytical column with UV detection, good separation and detection of the drugs was attained within a 10 min run time. Intra- and inter-assay precision RSD values were found to be less than 15% at the concentrations examined (0.1–20 μg/mL). The LOQ was found to be 0.1 μg/mL for each agent and the assay was found to generate a linear response up to 20 μg/mL.This low cost assay can accurately detect efavirenz and rifampicin concentrations within a clinically relevant concentration range using standard chromatography equipment, making it particularly applicable to resource-limited settings.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 56, Issue 4, 5 December 2011, Pages 785–791
نویسندگان
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