Explore protein molecular structure in endosperm tissues in newly developed black and yellow type canola seeds by using synchrotron-based Fourier transform infrared microspectroscopy

• Yellow canola seeds contained relatively higher percentage of a-helix and β-sheet than black canola seeds.
• Yellow canola seeds contained higher percentage of amide I and amide II area compared to the black canola seed.
• Not significant molecular structural differences in protein amide I and II region between yellow and black canola seed.

This study was conducted to characterize the protein molecular structure in endosperm tissues in newly developed black and yellow-type canola seeds by using synchrotron-based Fourier transform infrared microspectroscopy. The results showed that the yellow canola seeds contained relatively lower (P < 0.05) percentage of β-sheet and amide I and amide II area compared to the black-type canola seed. This might be an indication that the protein value of the yellow canola seeds as food or feed is different from that of the black canola seeds. The multivariate molecular spectral analyses (AHCA, PCA) showed that there were not significant molecular structural differences in the protein amide I and amide II fingerprint region (ca. 1720–1480 cm−1) between the yellow and the black-type of canola seed. It can be concluded that both the yellow and the black-seeded canola contain the same proteins but in different ratios.

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