کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1266063 1496849 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Systems Level Approach for Identification of Molecular Targets for Antimicrobial Intervention against Pseudomonas Aeruginosa, while Predicting Biofilm Formation
ترجمه فارسی عنوان
روش سطح سیستم برای شناسایی اهداف مولکولی برای مداخله ضدمیکروبی در برابر سودوموناس آئروژینوزا، به هنگام پیش بینی تشکیل بیوفیلم
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
چکیده انگلیسی

In this case study, we aimed at evaluating the suitability of genome-scale metabolic models to identify molecular targets that can potentially enhance antimicrobial effects of chemical preservatives against P. aeruginosa, while minimizing biofilm formation. For the case study, isothiazolinones were selected as a group of microbicides where their mechanism of action is well described in scientific literature. Target identification was carried out in several steps. First, we developed a computational model of P. aeruginosa metabolism under action of isothiazolinones. Action of sub-inhibitory concentrations of isothiazolinones was simulated based on extensive information on their mechanisms of action. Then, simulations of single and double gene deletion(s) were performed in silico to identify genes or combinations of genes that could be targeted to induce further reduction of bacterial growth rate. Finally, we assessed whether total or partial inhibition of these genes might activate biofilm formation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Procedia Food Science - Volume 7, 2016, Pages 113–116
نویسندگان
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