کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1369509 981781 2016 3 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Evaluation of phenylcyclopropylamine compounds by enzymatic assay of lysine-specific demethylase 2 in the presence of NPAC peptide
ترجمه فارسی عنوان
بررسی ترکیبات phenylcyclopropylamine با استفاده از روش آنزیمی demethylase 2 لیزین خاص در حضور پپتید NPAC
کلمات کلیدی
بازدارنده؛ demethylases لیزین خاص 1 و 2 (LSD1 و LSD2)؛ پپتید NPAC؛ demethylation هیستون H3K4
Inhibitor; Lysine-specific demethylases 1 and 2 (LSD1 and LSD2); NPAC peptide; Histone H3K4 demethylation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی

Lysine-specific demethylase 2 (LSD2) demethylates mono- and dimethylated Lys-4 of histone H3 (H3K4me1 and H3K4me2). NPAC protein is known to interact with LSD2 and promote its H3K4 demethylase activity. In this study, we established a demethylation assay system that utilizes recombinant LSD2 in the presence of a synthetic NPAC peptide. Several phenylcyclopropylamine (PCPA)-based inhibitors were examined for their LSD2 inhibitory activity in the LSD2 enzymatic assay with the NPAC peptide. The assay results showed that the PCPA derivatives, including NCD41, selectively inhibited LSD1 in preference to LSD2.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 26, Issue 4, 15 February 2016, Pages 1193–1195
نویسندگان
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