Structure of polysaccharide moiety of Pseudomonas xanthomarina KMM 1447T lipopolysaccharide

• Gram-negative bacteria Pseudomonas xanthomarina KMM 1447T produce two LPS species.
• Polysaccharide parts of two LPS have different structures.
• Structures of the two polysaccharides were established.
• One of them was found to contain a (2R,4R)-2,4-dihydroxypentanoic acid lactone.

The structural analysis of a polysaccharide moiety of Pseudomonas xanthomarina KMM 1447T lipopolysaccharide (LPS) was carried out. Mild acid degradation of LPS resulted in identification of two polysaccharides. The major one was built of β-D-GlcpNAcA residues amidated with L-Ala and Gly residues randomly. The minor polysaccharide was composed of branched tetrasaccharide repeating units constituted by two D-Galр, D-GalpNAc and ether of D-Glc with (2R,4R)-2,4-dihydroxypentanoic acid lactone (2R,4R-Dhpl): →3)-α-D-Galр-(1 → 3)-β-D-Galр-(1 → 3)-[β-D-Glcp4(2R,4R-Dhpl)-(1 → 4)]-β-D-GalpNAc-(1 → .

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