کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
16866 42619 2016 6 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Quantitative detection of selenate-reducing bacteria by real-time PCR targeting the selenate reductase gene
ترجمه فارسی عنوان
تشخیص کمی از باکتری های کاهش سلنات با استفاده از PCR در زمان واقعی، هدف قرار دادن ژن selenate reductase
کلمات کلیدی
سلنیت ردوکتاز؛ طراحی پرایمر؛ DMSO؛ راکتور بیوفیلم غشایی
Selenate reductase; Primer design; DMSO; Membrane biofilm reactor
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• Designed a primer set to target the selenate reductase (SerA).
• The primer set has high specificity and positively amplified environmental selenate reducing bacteria.

We designed a primer set to target selenate reductase (SerA) for detecting selenate reducing bacteria (SeRB). Our serA gene-based PCR primer set has high specificity in that it and positively amplified some SeRB, but not denitrifying bacteria (DB). Phylogenetic analysis of serA clone sequences of environmental samples from selenate-reducing membrane biofilm reactor (MBfR) biofilms showed that these sequences were closely grouped and had high similarity to selenate reductase gene sequences from SeRB Thauera selenatis and DB Dechloromonas; however, they were distant to other genes from dimethylsulfoxide (DMSO) enzyme family. Constructing a standard curve targeting the serA gene, we found that the good linearity for the qPCR assay when applied it to quantify SeRB in MBfR biofilms, and the gene copies of SeRB correlated well to the selenate removal percentages. Our results demonstrated the feasibility of using the serA gene-based PCR primer set to detect and quantify SeRB in environmental samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 85, April 2016, Pages 19–24
نویسندگان
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