کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1785782 1023394 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Self-assembled magnetic lamellar hydroxyapatite as an efficient nano-vector for gene delivery
ترجمه فارسی عنوان
هیدروکسی آپاتیت مغناطیسی به عنوان یک نانوذره کارآمد برای تحویل ژن
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم فیزیک ماده چگال
چکیده انگلیسی


• Magnetic lamellar hydroxyapatite (ML-HA) was synthesized by self-assembly process.
• The structure and morphology of ML-HA could be controlled by processing variables.
• ML-HA carried DNA molecules mainly via DNA intercalation into the gallery of ML-HA.
• ML-HA had higher transfection efficiency than the L-HA without magnetic particles.

Magnetic lamellar hydroxyapatite (ML-HA) nanoparticles were synthesized by a template-assisted self-assembly process. The as-prepared ML-HA nanoparticles self-assembled under different conditions were characterized by XRD, TEM, cytotoxicity assessment, and DNA-loading and transfection efficiency measurements. We found that the structure and morphology of ML-HA were controlled by self-assembly conditions. The ML-HA synthesized in this work exhibited good biocompatibility. The DNA-loading capacity and ζ-potential of ML-HA were much lower in comparison to bare lamellar HA (L-HA) without magnetic nanoparticles. Despite that, the ML-HA with good lamellar structure showed 47% higher transfection efficiency than L-HA. Results suggested that the ordered lamellar structure is a key factor in controlling transfection efficiency and magnetization is an effective way of improving the transfection efficiency of lamellar HA. Mechanisms were proposed to interpret these experimental results. It is demonstrated that the ML-HA may be a promising gene vector to deliver DNA into the cells effectively and safely.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Applied Physics - Volume 15, Issue 7, July 2015, Pages 811–818
نویسندگان
, , , , ,