کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1927806 1050260 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Iron deficiency anemia’s effect on bone formation in zebrafish mutant
ترجمه فارسی عنوان
اثر کم خونی فقر آهن بر تشکیل استخوان در جهش گورماهی
کلمات کلیدی
Fpn1؛ کم خونی کمبود آهن؛ تشکیل استخوان؛ گورخرماهی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

Iron is one of the essential elements of life. Iron metabolism is related to bone metabolism. Previous studies have confirmed that iron overload is a risk factor for osteoporosis. But the correlation between iron deficiency and bone metabolism remains unclear. Ferroportin 1 is identified as a cellular iron exporter and required for normal iron cycling. In zebrafish, the mutant of ferroportin 1 gene (fpn1), wehtp85c exhibited the defective iron transport, leading to developing severe hypochromic anemia. We used wehtp85c as a model for investigating iron deficiency and bone metabolism. In this study, we examined the morphology of the developing cartilage and vertebrae of the Wehtp85 compared to the wild type siblings by staining the larvae with alcian blue for cartilage and alizarin red for the bone. In addition, we evaluated the expression patterns of the marker genes of bone development and cell signaling in bone formation. Our results showed that wehtp85c mutant larvae exhibited the defects in bone formation, revealing by decreases in the number of calcified vertebrae along with decreased expression of osteoblast novel genes: alpl, runx2a and col1a1a and BMPs signaling genes in osteoblast differentiation: bmp2a and bmp2b. Our data suggest that iron deficiency anemia affects bone formation, potentially through the BMPs signaling pathway in zebrafish.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 475, Issue 3, 1 July 2016, Pages 271–276
نویسندگان
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