کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941586 1536900 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lack of mycothiol and ergothioneine induces different protective mechanisms in Mycobacterium smegmatis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Lack of mycothiol and ergothioneine induces different protective mechanisms in Mycobacterium smegmatis
چکیده انگلیسی


• MSH and ESH are involved in protection against metal stress in Mycobacterium smegmatis.
• MSH mutants have greater macromolecular damage while ESH mutants have lesser damage than wildtype.
• Different proteins associated with protection against oxidative stress are upregulated in mshC and egtA mutants.

Mycobacterium smegmatis contains the low molecular weight thiols, mycothiol (MSH) and ergothioneine (ESH). Examination of transposon mutants disrupted in mshC and egtA, involved in the biosynthesis of MSH and ESH respectively, demonstrated that both mutants were sensitive to oxidative, alkylating, and metal stress. However, the mshC mutant exhibited significantly more protein carbonylation and lipid peroxidation than wildtype, while the egtA mutant had less protein and lipid damage than wildtype. We further show that Ohr, KatN, and AhpC, involved in protection against oxidative stress, are upregulated in the egtA mutant. In the mshC mutant, an Usp and a putative thiol peroxidase are upregulated. In addition, mutants lacking MSH also contained higher levels of Coenzyme F420 as compared to wildtype and two Coenzyme F420 dependent enzymes were found to be upregulated. These results indicate that lack of MSH and ESH result in induction of different mechanisms for protecting against oxidative stress.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 8, December 2016, Pages 100–106
نویسندگان
, , , , ,