کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1957190 1057877 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mathematical Analysis and Quantification of Fluorescent Proteins as Transcriptional Reporters
ترجمه فارسی عنوان
آنالیز ریاضی و کمی فلورسنت پروتئین ها به عنوان رونویسی خبرنگاران ☆
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

Fluorescent proteins are often used as reporters of transcriptional activity. Here we present a mathematical characterization of a novel fluorescent reporter that was recently engineered to have a short half-life (∼12 min). The advantage of this destabilized protein is that it can track the transient transcriptional response often exhibited by signaling pathways. Our mathematical model takes into account the maturation time and half-life of the fluorescent protein. We demonstrate that our characterization allows transient transcript profiles to be inferred from fluorescence data. We also investigate a stochastic version of the model. Our analysis reveals that fluorescence measurements can both underestimate and overestimate fluctuations in protein levels that arise from the stochastic nature of biochemical reactions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 94, Issue 6, 15 March 2008, Pages 2017–2026
نویسندگان
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