Remnant lipoprotein size distribution profiling via dynamic light scattering analysis

• The first size distribution by dynamic light scattering of remnant lipoproteins: RLP
• Separation includes human serum albumin (HSA) and small, medium, and large RLP.
• Exploratory analyses reveal varied size distribution profiles in clinical subjects.
• Size distributions can assimilate the relationship of RLP size and atherogenicity.

BackgroundRemnant lipoproteins (RLP) are a metabolically derived subpopulation of triglyceride-rich lipoproteins (TRL) in human blood that are involved in the metabolism of dietary fats or triglycerides. RLP, the smaller and denser variants of TRL particles, are strongly correlated with cardiovascular disease (CVD) and were listed as an emerging atherogenic risk factor by the AHA in 2001.MethodsVarying analytical techniques used in clinical studies in the size determination of RLP contribute to conflicting hypotheses in regard to whether larger or smaller RLP particles contribute to CVD progression, though multiple pathways may exist.ResultsWe demonstrated a unique combinatorial bioanalytical approach involving the preparative immunoseparation of RLP, and dynamic light scattering for size distribution analysis.ConclusionsThis is a new facile and robust methodology for the size distribution analysis of RLP that in conjunction with clinical studies may reveal the mechanisms by which RLP cause CVD progression.

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