کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1971835 1538986 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Acute hyperthermic responses of heat shock protein and estrogen receptor mRNAs in rainbow trout hepatocytes
ترجمه فارسی عنوان
واکنش گرمازدگی حاد پروتئین شوک حرارتی و گیرنده استروژن mRNA ها در سلول های کبدی قزل آلای رنگین کمانی
کلمات کلیدی
پروتئین شوک حرارتی؛ گیرنده استروژن؛ Vitellogenin؛ هپاتوسيت؛ ماهی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

Heat shock proteins (HSPs) are induced upon elevated temperature in fishes. HSPs also function as molecular chaperones for cellular proteins, including steroid hormone receptors. Estrogen receptors (ERs) are critical for the hormone signaling necessary during the liver production of the yolk precursor protein vitellogenin in oviparous vertebrates. Considering the possible regulatory role of HSPs on the ER signaling pathway, the present study characterized the mRNA expression of all known isoforms of HSP70 (hsp70a, hsp70b), HSP90 (hsp90a1a, hsp90a1b, hsp90a2a, hsp90a2b, hsp90b1, hsp90b2), and ERs (erα1, erα2, erβ1, erβ2) in Rainbow Trout hepatocytes following an acute heat shock (1 h at 25 °C) compared to a control treatment (12 °C). The results showed that the mRNA levels of hsp70a, hsp70b, hsp90a1b, hsp90a2a, and hsp90b2 were significantly increased after heat shock, while erα1 mRNA levels were significantly reduced by this treatment. hsp90a1a, hsp90a2b, hsp90b1, erα2, erβ1 and erβ2 were unaffected by this acute hyperthermic treatment. Comparatively, the responses of the two hsp70 isoforms were much greater than the hsp90 isoforms. Acute heat shock treatment of hepatocytes followed by a 24 h exposure to 17β-estradiol (E2) exposure also resulted in decreased expression of erα1 mRNA, but not vitellogenin (vtg) mRNA. This study showed that some hsp70 and hsp90 isoforms display a robust response to an acute hyperthermic treatment in Rainbow Trout hepatocytes. Among the transcripts measured here, the erα1 isoform uniquely showed significantly decreased mRNA levels upon acute heat treatment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 201, November 2016, Pages 156–161
نویسندگان
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