کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1977223 1539284 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of perfluorooctane sulfonate on the immune responses and expression of immune-related genes in Chinese mitten-handed crab Eriocheir sinensis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effects of perfluorooctane sulfonate on the immune responses and expression of immune-related genes in Chinese mitten-handed crab Eriocheir sinensis
چکیده انگلیسی

Perfluorooctane sulfonate (PFOS) has been widely studied due to its global distribution, slow degradation, high bioaccumulation and toxicological effects on vertebrates. However, the potential toxicity of PFOS to crustaceans is little known. The present study investigated the effects of PFOS on the immune responses and expression of immune-related genes in the Chinese mitten-handed crab Eriocheir sinensis. Crabs were exposed to 0, 0.01, 0.1, 1.0 and 10 mg/L of PFOS, and sampled on 1, 4, 7, 14 and 21 days respectively. The total hemocyte count and lysozyme activity in PFOS-treated crab were significantly lower than in the control. The exposure to 10 mg/L of PFOS led to a marked inhibition in phenoloxidase and superoxide dismutase activities. At other PFOS levels, phenoloxidase and superoxide dismutase showed an initial increase and a subsequent decrease over time. The alkaline and acid phosphatase activities were stimulated in 10 mg/L PFOS until 21 days. The mRNA expression of immune related genes including hepatopancreas-specific C-type lectin and prophenoloxidase activating factors were up-regulated after the exposure to the concentrations of 1 and 10 mg/L of PFOS, while the expression of lysozyme gene was up-regulated only in the crab exposed to 0.1 mg/L PFOS. The results demonstrate that the high dose of PFOS leads to immune toxicity and the hepatopancreas is a major target organ for PFOS accumulation and immunotoxicity. Hemocyte counts, phenoloxidase and acid phosphatase are useful biomarkers for the risk assessment of PFOS toxicity to crustaceans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volumes 172–173, June–July 2015, Pages 13–18
نویسندگان
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