کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1985822 1540235 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultrasonic extraction, antioxidant and anticancer activities of novel polysaccharides from Chuanxiong rhizome
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Ultrasonic extraction, antioxidant and anticancer activities of novel polysaccharides from Chuanxiong rhizome
چکیده انگلیسی


• Three novel polysaccharides were obtained from L. chuanxiong rhizome.
• The extraction condition was optimized using orthogonal experimental design.
• The polysaccharides showed potent in vitro antioxidant and anticancer activities

Ultrasonic-assisted extraction technology was employed to prepare Ligusticum chuanxiong Hort polysaccharide. Single factor test and orthogonal experimental design were used to optimize the extraction conditions. The results showed that the optimal extraction conditions consisted of ultrasonic temperature of 80 °C, ultrasonic time of 40 min and water to raw material ratio of 30 mL/g. Three novel polysaccharides fractions, LCX0, LCX1 and LCX2, were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography. The molecular weight and monosaccharide composition of three LCX polysaccharides fractions were analyzed with gel permeation chromatography (GPC) and HPLC analysis, respectively. Furthermore, the antioxidant and in vitro anticancer activities of the polysaccharides were investigated. Compared with LCX0, LCX2 and LCX1 showed relative higher antioxidant activity and inhibitory activity to the growth of HepG2, SMMC7721, A549 and HCT-116 cells. It is suggested that the novel polysaccharides from rhizome of L. chuanxiong could be promising bioactive macromolecules for biomedical use.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 85, April 2016, Pages 277–284
نویسندگان
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