کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1988697 1540445 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development
ترجمه فارسی عنوان
بیان diphosphohydrolase3 ecto نوکلئوزیدی تری فسفات (NTPDase3) در مغز رت ماده در طول توسعه پس از زایمان
کلمات کلیدی
ATP، آدنوزین تری فسفات؛ ADP، آدنوزین دی فسفات؛ در قوس دار، هسته های کمانی؛ CHP، شبکه کوروئید؛ CM، مرکزی هسته تالاموس داخلی؛ D3V، بطن سوم؛ DMC، بخشی جمع و جور از هسته هیپوتالاموس dorsomedial؛ DMD، هسته هیپوتالاموس dorsomedial؛ D
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• NTPDase3 is the a membrane-bound ecto-enzyme which hydrolyzes extracellular ATP.
• Developmental expression of NTPDase3 was analyzed in female rat brain at several postnatal ages (PD7-PD90).
• Faint NTPDase3-imunoreactivity (ir) was observed at PD7 and gradually increased in PD15 and PD20 at clusters of hypothalamic neurons.
• NTPDase3-ir fibers were first observed at PD20.
• NTPDase3- ir fiber density and the varicose appearance increased until the adulthood.

Nucleoside triphosphate diphosphohydrolase3 (NTPDase3) is membrane-bound ecto-enzyme which hydrolyzes extracellular ATP, thus modulating the function of purinergic receptors and the pattern of purinergic signaling. Here we analyzed the developmental expression of NTPDase3 in female hypothalamus, cerebral cortex and hippocampal formation at different postnatal ages (PD7-PD90) by qRT-PCR and immunohistochemistry. In hypothalamus and hippocampus, a similar developmental profile was seen: NTPDase3 gene expression was stable during postnatal development and increased in adults. In the cortex, upregulation of NTPDase3 mRNA expression was seen at PD15 and further increase was evidenced in adults. Immunohistochemical analysis at PD7 revealed faint neuronal NTPDase3 localization in a dorsal hypothalamus. The immunoreactivity (ir) gradually increased in PD15 and PD20, in clusters of cells in the lateral, ventral and dorsomedial hypothalamus. Furthermore, in PD20 animals, NTPDase3-ir was detected on short fibers in the posterior hypothalamic area, while in PD30 the fibers appeared progressively longer and markedly varicose. In adults, the strongest NTPDase3-ir was observed in collections of cells in dorsomedial hypothalamic nucleus, dorsal and lateral hypothalamus and in several thalamic areas, whereas the varicose fibers traversed entire diencephalon, particularly paraventricular thalamic nucleus, ventromedial and dorsomedial hypothalamic nuclei, the arcuate nucleus and the prefornical part of the lateral hypothalamus. The presumably ascending NTPDase3-ir fibers were first observed in PD20; their density and the varicose appearance increased until the adulthood. Prominent enhancement of NTPDase3-ir in the hypothalamus coincides with age when animals acquire diurnal rhythms of sleeping and feeding, supporting the hypothesis that this enzyme may be involved in regulation of homeostatic functions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chemical Neuroanatomy - Volume 77, November 2016, Pages 10–18
نویسندگان
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