کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1993462 1064669 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-microfluidic methods for imaging live C. elegans
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Non-microfluidic methods for imaging live C. elegans
چکیده انگلیسی

There are many challenges to live Caenorhabditis elegans imaging including the high motility of the animals and sustaining their viability for extended periods of time. Commonly used anesthetics to immobilize the C. elegans for imaging purpose prevents feeding of the animals and can cause cellular physiologic changes. Here we present three adapted or novel methodologies to image live C. elegans over different imaging microscopy equipment to allow for visualization of animals by DIC and fluorescence without the use of microfluidic technologies. The methods present here use common microscopy consumables and equipment found in many imaging core facilities and can be easily adapted to fit on multiple microscopy systems.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 68, Issue 3, 1 August 2014, Pages 542–547
نویسندگان
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