کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
20082 43156 2016 5 صفحه PDF ندارد دانلود کنید
عنوان انگلیسی مقاله
Production of γ-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system
ترجمه فارسی عنوان
تولید γ-cyclodextrin توسط Bacillus cereus cyclodextrin glycosyltransferase با استفاده از Bioconversion استخراج شده در سیستم دو فازی آبی پلیمری نمک
کلمات کلیدی
سیستم دو فازی آبی؛ باسیلوس سرئوس؛ سیکلوکودکسترین؛ گلیکوزیل ترانسفراز سیکلوکودکسترین؛ بیو Conversion Extractive
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• ATPS extractive bioconversion composed by PEG and potassium phosphates solution.
• The ATPS was optimized and ATPS extractive bioconversion was then applied.
• Phase composition and phase concentration influenced the partition behavior of γ-CD.
• The γ-CD concentration of 1.60 mg/mL with 19% concentration ratio was recovered after 1 h extractive bioconversion process.

Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme derived from Bacillus cereus was evaluated using polyethylene glycol (PEG)/potassium phosphate based on ATPS. The optimum condition was attained in the ATPS constituted of 30.0% (w/w) PEG 3000 g/mol and 7.0% (w/w) potassium phosphate. A γ-CD concentration of 1.60 mg/mL with a 19% concentration ratio was recovered after 1 h bioconversion process. The γ-CD was mainly partitioned to the top phase (YT = 81.88%), with CGTase partitioning in the salt-rich bottom phase (KCGTase = 0.51). Repetitive batch processes of extractive bioconversion were successfully recycled three times, indicating that this is an environmental friendly and a cost saving technique for γ-CD production and purification.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 6, June 2016, Pages 692–696
نویسندگان
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