کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2008988 | 1541771 | 2016 | 5 صفحه PDF | دانلود رایگان |
• Motility rate and duration of spermatozoa decreased with exposure to cypermethrin.
• GSH-Px activity of spermatozoa increased significantly.
• MDA and GSH levels slightly increased while CAT activity decreased significantly.
There is limited information on the scientific literature about the effect of in vitro exposure of fish sperm to pesticides. In vitro effect of cypermethrin on sperm quality and oxidative stress has not yet been fully investigated. Therefore, the effects of cypermethrin, a type II pyrethroid insecticide, on quality and oxidative stress of spermatozoa were examined in vitro. To explore the potential in vitro toxicity of cypermethrin, fish spermatozoa were incubated with different concentrations of cypermethrin (1.025, 2.05 and 4.1 μg/l) for 2 h. The motility rate and duration of sperm were determined after exposure to cypermethrin. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and malondialdehyde (MDA) in spermatozoa were analyzed for determination of oxidant and antioxidant balance. Our results indicated that spermatozoa motility and duration significantly decreased with exposure to cypermethrin. Additionally, activity of GSH-Px (P < 0.05) and MDA and GSH levels increased in a concentration-dependent manner while CAT activity decreased (P < 0.05). Consequently, the oxidant and antioxidant status and sperm quality were affected by quantitative changes and different concentrations of cypermethrin.
Fish are affected by residues of cypermethrin in water and sediment samples due to discharge into waters of the majority of pollutants. Sperm cells in most fish species release into water and directly expose to pollutants prior to fertilization. Reactive oxygen species (ROS) generate in spermatozoa due to contain highly polyunsaturated fatty acids (PUFA) in their membranes and a largely lack of protective cytoplasmic enzymes. Increased lipid peroxidation of polyunsaturated fatty acids in the sperm plasma membrane can reduce the fluidity of the membrane and lead to loss of cell function, thereby reducing sperm motility and duration when spermatozoa are exposed to cypermethrin.Figure optionsDownload as PowerPoint slide
Journal: Pesticide Biochemistry and Physiology - Volume 128, March 2016, Pages 63–67