کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
20099 43157 2016 5 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae
ترجمه فارسی عنوان
غلظت نانو گلوتاتیون خارج سلولی تولید شده توسط مهندسی ساکارومایسس سرویزیه
کلمات کلیدی
گلوتاتیون غیر سلولی؛ Saccharomyces cerevisiae؛ مصنوعی تعریف شده متوسط؛ عصاره گیاه پپتون دگزستروز؛ نانوفیلتراسیون
Extracellular glutathione; Saccharomyces cerevisiae; Synthetic defined medium; Yeast extract peptone dextrose medium; Nanofiltration
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

This study aimed to optimize extracellular glutathione production by a Saccharomyces cerevisiae engineered strain and to concentrate the extracellular glutathione by membrane separation processes, including ultrafiltration (UF) and nanofiltration (NF). Synthetic defined (SD) medium containing 20 g L−1 glucose was fermented for 48 h; the fermentation liquid was passed through an UF membrane to remove macromolecules. Glutathione in this permeate was concentrated for 48 h to 545.1 ± 33.6 mg L−1 using the NF membrane; this was a significantly higher concentration than that obtained with yeast extract peptone dextrose (YPD) medium following 96 h NF concentration (217.9 ± 57.4 mg L−1). This higher glutathione concentration results from lower cellular growth in SD medium (final OD600 = 6.9 ± 0.1) than in YPD medium (final OD600 = 11.0 ± 0.6) and thus higher production of extracellular glutathione (16.0 ± 1.3 compared to 9.2 ± 2.1 mg L−1 in YPD medium, respectively). Similar fermentation and membrane processing of sweet sorghum juice containing 20 g L−1 total sugars provided 240.3 ± 60.6 mg L−1 glutathione. Increased extracellular production of glutathione by this engineered strain in SD medium and subsequent UF permeation and NF concentration in shortend time may help realize industrial recovery of extracellular glutathione.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 1, January 2016, Pages 96–100
نویسندگان
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