کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2024360 1542588 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protease encoding microbial communities and protease activity of the rhizosphere and bulk soils of two maize lines with different N uptake efficiency
ترجمه فارسی عنوان
جوامع میکروبی رمزگذاری پروتئاز و فعالیت پروتئاز ریزوسفر ها و خاک های بخش اعظم دو خط ذرت با کارایی جذب N مختلف
کلمات کلیدی
کارایی مصرف نیتروژن؛ ریزوسفر؛ فعالیت پروتئاز؛ ژن کدکننده پروتئاز؛ توالی روشنگر؛ PCR-DGGE
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش خاک شناسی
چکیده انگلیسی


• Rhizosphere and bulk soil from maize plants with different NUEs are studied.
• Soil proteolytic communities are studied using DGGE, Illumina sequencing and qPCR.
• Higher NUE plant harbor higher abundance and diversity of proteolytic communities.
• Most of the neutral protease gene sequences belong to uncultured bacterial species.
• Rhizosphere effect due to different NUEs is stronger on apr gene than on npr gene.

This study was carried out to understand the interplay of plant Nitrogen Utilizing Efficiency (NUE) with protease activity and microbial proteolytic community composition in the rhizosphere and bulk soils. Protease activity, diversity and abundance of protease genes (using DGGE and qPCR respectively of two key bacterial protease encoding genes: alkaline metallo-peptidase (apr) and neutral-metallopeptidases (npr)) were monitored in both rhizosphere and bulk soils from two maize in-bred lines L05 and T250 with higher and lower NUE respectively, using a rhizobox approach. Illumina sequencing was employed to assess the diversity of proteolytic communities encoding for the above-mentioned protease genes. Our results show higher enzyme activity, higher abundance and diversity of proteolytic genes in L05 maize rhizosphere, with higher NUE than in T250 maize rhizosphere.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Soil Biology and Biochemistry - Volume 96, May 2016, Pages 176–179
نویسندگان
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