کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2027622 | 1542700 | 2016 | 6 صفحه PDF | دانلود رایگان |
• Postnatal dexamethasone (DEX) induced programmed hypertension in later life.
• Maternal melatonin therapy prevented DEX-induced programmed hypertension.
• Postnatal DEX decreased renal MT2 protein level, which melatonin prevented.
• Melatonin therapy increased levels of RORα protein and melatonin in the kidney.
• Postnatal DEX-induced programmed hypertension is related to melatonin receptors.
Adulthood hypertension can be programmed by glucocorticoid exposure in early life. We found that maternal melatonin therapy prevents postnatal dexamethasone (DEX)-induced programmed hypertension. Melatonin acts through specific receptors, including MT1 and MT2 membrane receptors, and retinoid related orphan nuclear receptors of the RZR/ROR family. Thus we tested whether postnatal DEX-induced hypertension is related to changes of melatonin receptors in the kidney and heart, which was preserved by maternal melatonin therapy. Male neonates were assigned to four groups (n = 6–8/group): control, DEX, control + melatonin (MEL), and DEX + MEL. Male rat pups were injected i.p. with DEX on d 1 (0.5 mg/kg BW), d 2 (0.3 mg/kg BW), and d 3 (0.1 mg/kg BW) after birth. Melatonin was administered in drinking water (0.01%) during the lactation period. We found DEX group developed hypertension at 16 weeks of age, which melatonin therapy prevented. Postnatal DEX treatment increased mRNA expression of MT1 and MT2, while decreased RORα and RZRβ in the kidney. These changes were prevented by melatonin therapy. Postnatal DEX decreased protein level of MT2 in the kidney, which was attenuated by melatonin therapy. Renal protein level of RORα was higher in DEX + MEL group compared to control and DEX group. Renal melatonin level was higher in the MEL and DEX + MEL groups compared to control. We concluded that melatonin therapy has long-term protection on postnatal DEX-induced programmed hypertension, which is associated with regulation on melatonin receptors in the kidney. Our findings would offer potential therapeutic approaches to prevent programmed hypertension in premature baby receiving glucocorticoids.
Journal: Steroids - Volume 108, April 2016, Pages 1–6