کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2047896 | 1074044 | 2012 | 8 صفحه PDF | دانلود رایگان |
Crucial to organellogenesis was the development of membrane translocases responsible for delivering proteins to new cellular compartments. This investigation examines the Trichomonas vaginalis hydrogenosome, a mitochondrially derived organelle. We identify an expanded family of putative β-barrel proteins (THOM A–I) comprising nine related sequences. Sub-cellular localisation by immunofluorescence and biochemical fractionation is consistent with THOMs being localised to the hydrogenosomal membrane. Native gel electrophoresis and chemical cross-linking support the ability of THOM proteins to be components of membrane-bound oligomeric protein complexes, consistent with a role in protein translocation.
► Trichomonas has an expanded family of Tom40-like proteins.
► Tom40 candidates are visualised under confocal microscopy to hydrogenosomal membranes.
► Molecular biology approaches localise recombinant protein to hydrogenosomal membranes.
► Tom40 homologues form complexes in membranes.
Journal: FEBS Letters - Volume 586, Issue 22, 16 November 2012, Pages 4038–4045