کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2047896 1074044 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Localisation of a family of complex-forming β-barrels in the T. vaginalis hydrogenosomal membrane
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Localisation of a family of complex-forming β-barrels in the T. vaginalis hydrogenosomal membrane
چکیده انگلیسی

Crucial to organellogenesis was the development of membrane translocases responsible for delivering proteins to new cellular compartments. This investigation examines the Trichomonas vaginalis hydrogenosome, a mitochondrially derived organelle. We identify an expanded family of putative β-barrel proteins (THOM A–I) comprising nine related sequences. Sub-cellular localisation by immunofluorescence and biochemical fractionation is consistent with THOMs being localised to the hydrogenosomal membrane. Native gel electrophoresis and chemical cross-linking support the ability of THOM proteins to be components of membrane-bound oligomeric protein complexes, consistent with a role in protein translocation.


► Trichomonas has an expanded family of Tom40-like proteins.
► Tom40 candidates are visualised under confocal microscopy to hydrogenosomal membranes.
► Molecular biology approaches localise recombinant protein to hydrogenosomal membranes.
► Tom40 homologues form complexes in membranes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 586, Issue 22, 16 November 2012, Pages 4038–4045
نویسندگان
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