کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2058831 1543978 2016 3 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Enhancement of human plasma glucosylceramide assay sensitivity using delipidized plasma
ترجمه فارسی عنوان
افزایش حساسیت سنجش glucosylceramide پلاسمای انسانی با استفاده از پلاسمای delipidized
کلمات کلیدی
Glucosylceramide؛ Globotriaosylceramide؛ طیف سنجی جرمی کروماتوگرافی مایع پشت سر هم (LC / MS / MS)؛ Delipidized پلاسما؛ حساسیت؛ روش علاوه بر استاندارد
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی

Glucosylceramide (GL-1) level in human has been considered as a surrogate biomarker for enzyme replacement and substrate reduction therapies (ERT and SRT) for Gaucher and Fabry patients. Due to the high endogenous level of GL-1 in human plasma, it is difficult to achieve the analytical sensitivity of plasma GL-1 below the normal endogenous level (1.7 μg/mL to 6.6 μg/mL) when using the standard addition method and regular plasma matrix for standard curve. A high sensitivity plasma GL-1 assay with LLOQ at 0.1 μg/mL was developed and validated using delipidized plasma so that patient plasma concentrations that are below normal reference range can be measured accurately. The normal reference range was established from 120 healthy donors using this developed new method. Twenty-three Fabry patient plasma samples including baseline and post-investigation drug treatment samples were measured. All post-treatment samples showed GL-1 concentration below 2.0 μg/mL, indicating the utility of the reported high sensitivity assay using delipidized plasma for monitoring the plasma GL-1 biomarker level in patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Genetics and Metabolism Reports - Volume 8, September 2016, Pages 77–79
نویسندگان
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