کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2072503 | 1544715 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effectiveness of glucose-methanol extender for cryopreservation of Huso huso spermatozoa
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present approach was designed to evaluate the methanol-glucose extender effects on sperm cryopreservation in beluga sturgeon, Huso huso. Sperm quality was examined by measuring post-thaw sperm motility and fertilizing rate at hatching stage. We first tested the effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30Â M) in a methanol extender on post-thaw sperm motility. The optimal cryopreservation conditions were found to be 0.2Â M glucose in the extender. Then, motility and fertilization rates of sperm cryopreserved with 0.2Â M glucose and 10% methanol (GM) were compared to Tris-sucrose-KCl in 10% methanol extender (TSKM). Additionally, sperm motility and fertilizing ability in relation to 15 and 30Â min equilibration in GM extender before and after cryopreservation were measured. Higher post-thaw sperm motility duration and percentage as well as fertilization rate were obtained with the GM extender when compared to TSKM extender. Equilibration of sperm in extender did not affect the motility quality of either fresh-diluted or frozen/thawed sperm, while fertilization rate showed a significant decline alone after 30Â min of post-thaw storage. Our results indicated that the use of a simple extender consisting of 0.2Â M glucose in 10% methanol can be an alternative cryopreservation method to those previously described for sturgeons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 162, November 2015, Pages 37-42
Journal: Animal Reproduction Science - Volume 162, November 2015, Pages 37-42
نویسندگان
Mohammad Sadegh Aramli, Karim Golshahi, Rajab Mohammad Nazari, Salim Aramli, Ashkan Banan,