کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2167238 | 1549413 | 2012 | 6 صفحه PDF | دانلود رایگان |
Human peripheral blood monocytes were stimulated with lipopolysaccharide at different temperatures (34, 37 and 40 °C) and TNF-α, IL-1β and NO production was measured. Levels of TNF-α mRNA and IL-1β mRNA were measured by RT-PCR. Phagocytic activity of LPS-stimulated monocytes in terms of bacterial uptake and intracellular bacterial killing was checked at different conditions in vitro. Early elevation of TNF-α, IL-1β and NO production was found in LPS-stimulated monocytes that incubated at 40 °C followed by cells that incubated at 37 °C and lowest level was detected at 34 °C. Similar results were observed in the phagocytic activity. Expression of TNF-α mRNA and IL-1β mRNA was observed as early as 30 min post exposure to LPS in all studied temperatures and these, decreased sharply after 12 h post exposure to LPS in LPS-stimulated monocytes that incubated at 40 °C only. This report describes the striking effects of incubation temperature on activity of LPS-stimulated monocytes.
► Highest pro-inflammatory cytokines produced by LPS-stimulated monocytes incubated at 40 °C.
► TNF-α mRNA and IL-1β mRNA expression were abundant in monocytes stimulated with LPS and incubated at 40 °C.
► Maximum phagocytic activity of LPS-stimulated monocyte was observed when it incubated at 40 °C.
► The high efficiency of LPS-stimulated monocytes to produce NO was found at 40 °C.
► Incubation temperature modulated LPS-stimulated monocytes activity in vitro.
Journal: Cellular Immunology - Volume 275, Issues 1–2, January–February 2012, Pages 55–60