کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2167284 | 1092323 | 2011 | 9 صفحه PDF | دانلود رایگان |
Thymosin-β4 (Tβ4) is a G-actin sequestering peptide involved in regeneration and remodeling of injured tissues. In this work, we have designed and synthesized three peptide sequences containing the N-terminus (TYB4-n), the central part (TYB4-i) or the C-terminus (TYB4-c) of Tβ4. All fragments are overlapping on the main central binding actin site. After a structural characterization, we have evaluated in vitro and in vivo their pro-angiogenic effects. The results of this study have shown that: (i) each fragment reproduces the native conformation; (ii) Tβ4-derived peptides exert both in vitro and in vivo pro-angiogenic effects; (iii) their in vitro effect seem to be related to the activation of several signaling pathways and is positively modulated by the N-terminus of Tβ4.
► Thymosin-β4 (Tβ4) is a G-actin sequestering peptide involved in regeneration and remodeling of injured tissues.
► TYB4-n, TYB4-i, and TYB4-c have been evaluated in vitro and in vivo for pro-angiogenic effects.
► These effects are related to the activation of several signaling pathways and are modulated by the N-terminus of Tβ4.
Journal: Cellular Immunology - Volume 271, Issue 2, 2011, Pages 299–307